日志
细胞计数板的使用指南
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Directions for Use
Bright-Line / Dark-Line Counting Chambers
Catalog Numbers: 3100, 3110, 3200, 3500, 1490, 1492, 1475, &
1483
Usage: Cell Counts
Cell Depth: 0.100mm +/- 2% (1/10mm)
Volume: 0.1 Microliter
Ruling
Pattern: Improved Neubauer, 1/400 Square mm
Rulings cover 9 square millimeters. Boundary lines of the Neubauer ruling are the center lines of the groups of three. (These are indicated in the illustration below.) The central square millimeter is ruled into 25 groups of 16 small squares, each group separated by triple lines, the middle one of which is the boundary. The ruled surface is 0.10mm below the cover glass, so that the volume over each of the 16 small squares is .00025 cubic mm.
Neubauer
Ruling
The number of cells per cubic millimeter =
Number of cells
counted per square millimeter X dilution (if used) X 10
The number of cells per milliliter =
Number of cells counted per
square millimeter X dilution (if used) X 10,000
One (1) Milliliter = 1000 cubic millimeters (cu mm)
One (1) Microliter (ul) = One (1) cubic millimeter (cu mm)
To clean the counting chamber: After completing the count, remove the cover
glass and clean the counting chamber with water or a mild cleaning solution (10%
solution of bleach). Dry the counting chamber with a soft cloth or wipe, or
rinse with acetone.
